The human body reacts to infection and disease by increasing or decreasing the levels of cytokines in the blood. The levels of cytokines can be raised abnormally in the blood by various diseases and conditions. To detect these cytokine abnormalities, cytokine analysis is performed. Cytokine analysis detects the levels of certain key immune system chemicals called cytokines produced by various cells, including lymphocytes, monocytes, and macrophages. Detecting and identifying the level of these inflammatory proteins provides doctors with great insight into many conditions that go undiagnosed due to their nonspecific symptoms.
Various cytokines have been discovered, especially in the last several decades. These numerous cytokines act on a wide range of cells, allowing communication between those cells. The interactions between the cytokines provide an opportunity for the immune system to respond to pathogens by either fighting them or eliminating them. Cytokines also control various other functions. The discovery of numerous cytokines and their interactions has broadened the understanding of how the immune system fights disease, contributing to autoimmune disorders.
What are Cytokines
In immunology, a cytokine is a small protein used for cell-to-cell communication. It tells your body’s immune cells about things like infections and other abnormalities. There are different types of cytokines, and they each have various roles in the immune system. To make sure that infections are fought efficiently, the body needs to tell the difference between foreign invaders (like bacteria or viruses) and friendly human cells. This is done with cytokines, which activate certain types of white blood cells to attack the invader.
Cytokines cause cell activation, division, apoptosis, or movement. They serve as autocrine, paracrine, or endocrine messengers. Cytokines produced by leucocytes and which mainly affect other white cells are called interleukins. Cytokines that have a chemoattractant activity are known as chemokines. Those that result in differentiation and proliferation of stem cells are known as colony-stimulating factors.
Some cytokines also maintain a role in defense and interfere with viral replication and, as such, are called interferons. Immuno assays are the methods used in the detection of cytokines. Enzyme-linked immunosorbent assay (ELISA) is the commonly used form of immunoassay to detect and analyze Cytokines. There are several cytokines, but we would be looking at these IL 10 antibodies, Iba1 antibody, IL 1b antibody.
Anti-Interleukin-10 IL10 Antibody
This antibody reacts with rat cell tissues as its host with each vial containing 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. IL 10 antibody should be Stored at -20˚C for one year from the date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. As much as possible, you should avoid repeated freeze-thaw cycles. Il10 antibody is Polyclonal in clonality and has no cross-reactivity with other proteins. Ok10 antibody is validated on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive and negative samples to ensure specificity and high affinity.
Western blot analysis of IL10 using an anti-IL10 antibody (RP1016) (https://www.bosterbio.com/anti-rat-il-10-antibody-rp1016-boster.html)
The Anti Lba1 is also known as AIF1 Antibody Picoband. The Anti-Lba1 has Rabbit as its host cell tissue with each vial containing 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Anti-Lba1 is Polyclonal in clonality, with no cross-reactivity with other proteins samples. Anti-Lba1 has a booster validation on WB, IHC, ICC, Immunofluorescence, and ELISA with renowned positive and negative samples to make sure specificity and high affinity. Reconstituting of Anti-Lba1 requires you to add 0.2ml of distilled water, yielding a concentration of 500ug/ml. Anti-Lba1 should be stored at -20˚C for one year from the date of receipt. After reconstitution, it should be stored at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Western blot analysis of Iba1 using anti-Iba1 antibody (A01394) IHC analysis of Iba1 using an anti-Iba1 antibody (A01394)
IL 1b Antibody
IL 1b antibody is also known as Anti-IL1 Beta picoband. The IL 1b antibody has a size of 100ug/vial with each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. The IL 1b antibody has a Lyophilized form and has Rabbits as its host; the antibody has no cross-reactivity with other protein samples. IL 1b Antibody has a booster validity on WB, IHC, ICC, Immunofluorescence, and ELISA with renowned positive and negative samples to make sure specificity and high affinity. To reconstitute IL 1b antibody, you should add 0.2ml of distilled water to yield a concentration of 500ug/ml.
Western blot analysis of IL1 beta using an anti-IL1 beta antibody (A00101-1)
What is the Importance of Cytokines Detection and Analysis
Cytokines are very important in the communication between cells in the body; Cytokines serve as regulators of immune response in the body during analysis. They work within the immune system and between the immune system and other complex cellular networks within different tissues in the body.